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This site needs JavaScript to work properly. tration) for accomplishing high yields of β-galactosidase activity from Lactobacillus acidophilus ATCC 4356, bacteria regarded as safe for human consumption, were investigated. In this study, we developed a multiplex PCR method to distinguish six closely related species in the Lactobacillus acidophilus group (L. gasseri, L. acidophilus, L. helveticus, L. jensenii, L. crispatus, and L. gallinarum), which is based on species-specific primer sets. Beneficial microbes 2009;1(1):11–29. Lactobacillus acidophilus has been used as a probiotic, or "friendly bacteria." It comes as a capsule to take by mouth as directed by your physician. M, size marker(bp); 1. Contraindicated in patients with known hypersensitivity and intestinal damage or infection. Gancheva A, Pot B, Vanhonacker K, Hoste B, Kersters K. Syst Appl Microbiol. In situ preservation is more economical as it does not include the cost of purification of bacteriocins. How to cite this article: Hassan Pyar and Kok-Khiang Peh, 2014. All primer sets were pooled for the multiplex PCR reaction. Schillinger U, Yousif NM, Sesar L, Franz CM.
Altogether, 86 genomes of 9 Lactobacillus species from the National Center of Biotechnology Information (NCBI) database were compared to detect species-specific genes and design six species-specific primer sets. This site does not host or receive funding from advertising. Comparison of six species-specific primer…. Trends Food Sci Technol 2004;15(2):67–78. Always seek the advice of a qualified physician for medical diagnosis and treatment.Full Disclaimer, Advertise with us | Medindia Copyright | Privacy Policy | Terms of Use © All Rights Reserved 1997 - 2020. Harvesters October/November/December 2020.  |   | 
Lactobacillus acidophilus is a commercially significant bacterial probiotic, originally isolated from the human gastrointestinal tract and designated Bacillus acidophilus in 1900. Many Lactobacillus species are frequently isolated from dairy products, animal guts, and the vaginas of healthy women.

This is mainly done by breaking down the sugar lactose in milk. Each type of probiotic supplement — and each strain of each type — can work in different ways. Curr Microbiol. 2009 Jan;72(1):93-100. doi: 10.4315/0362-028x-72.1.93. -, Dicks L, Botes M. Probiotic lactic acid bacteria in the gastro-intestinal tract: health benefits, safety and mode of action. This site complies with the HONcode standard for trustworthy health information: verify here. 2007 Mar;17(3):490-5. They are also called L. acidophilus. 10.1007/s00253-016-8005-7  |  These results indicated that species-specific primer sets designed from the genome comparison could identify one strain within the six Lactobacillus species by a single PCR reaction. Fig 2. They make lactic acid by breaking down carbohydrates. Lactisyn 10ms / 10ms / 490ms / 490ms / 1amp - 1 Ampoule Injection (Lactobacillus Acidophilus) drug information. -, Perdigón G, Fuller R, Raya R. Lactic acid bacteria and their effect on the immune system. See this image and copyright information in PMC. Gel electrophoresis results of gradient…. The PCR conditions of the individual primer sets were optimized via gradient PCR methods. Fig 1. Appl Microbiol Biotechnol 2017;101(1):35–45. HHS

Curr Issues Intestinal Microbiol 2001;2(1):27–42. J Mol Microbiol Biotechnol 2007;13(4):194–199. De Vuyst L, Leroy F. Bacteriocins from lactic acid bacteria: production, purification, and food applications.

When identifying a single strain, the optimized multiplex PCR method can specifically detect one of the six species, but no band was amplified at least from the other Lactobacillus and Enterococcus species.

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